In silico and in vitro studies of novel cyanoacrylamides incorporating pyrazole moiety against breast and prostate carcinomas

Abstract We report novel cyanoacrylamide derivatives bearing the pyrazole moiety. The molecular structures of prepared cyanoacrylamides were confirmed by different spectral tools such as NMR, IR, and elemental analyses. anticancer effect all newly was studied against four cancer cell lines (HEPG2, MCF7, PACA2, PC3) well normal line (BJ1). best cytotoxic shown PC3, where compounds 5f 5i revealed promising IC 50 values (11.7 66.8 µM) respectively compared to doxorubicin (43.8 µM). In addition, effective screened a BJ1 line, which showed selectivity PC3 moderate toward MCF7 cells. docking study affinities 5c 5d STAT1 protein compound KRAS with energy scores. subsequent experiments on 5b , . Quantitative Real-time-PCR that expression RBL2 STAT2 genes down-regulated in treated cells much lower than other samples. Also, level SMAD determined, significant down-regulation them percentages DNA damage raised significantly samples negative control, highest for Graphical